The default software and middleware stacks can be extended thanks to enhanced STM32Cube Expansion Packages. STMicrolectronics or STMicrolectronics' partner packages can be downloaded directly from a dedicated package manager available within STM32CubeMX, while the other packages can be installed from a local drive.
A complete list of primers used is supplied as Supplementary Data 7. In the first phase of mapping and analysis, 30 polymorphic simple sequence repeat (SSR) markers and six previously developed sequence-tagged site (STS) markers41 (Supplementary Fig. 1) were used to genotype the population of 170 DH lines, and a genetic linkage map of chromosome 3D was assembled as previously described21. Over 300 bin-mapped expressed sequence tagged (EST) sequences that were known to map to the most distal deletion bin of 3DL (containing the Stb16q locus) were then downloaded and used to develop PCR primers, which were tested on TA4152-19 and ND495 for polymorphism and used to generate a genetic linkage map. Regression mapping was used to identify markers significantly associated with STB resistance (Supplementary Fig. 1). In a second phase, we used the ITMI (175 DH, 4,209 markers + 416,856 GBS markers) reference genetic maps22,42 to increase further the number of markers at the Stb16q locus. Polymorphic SSR markers barc323 and cfd9 flanking Stb16q on the DH TA4152-19 × ND495 population were used to genotype the ITMI population and analyzed using the GenMapper software. Using the RQTL package and Carthagene43, we constructed draft 3D linkage maps that included the Stb16q flanking markers.
More generally, although the method was specifically developed to inform and test situational action theory, its focus on situational causes makes it a potentially useful instrument for many other theoretical frameworks. The routine activity perspective (Cohen and Felson ; Felson and Boba ), the routine activity theory of individual deviancy (Osgood et al. ), situational crime prevention (Clarke , ) and crime pattern theory (Brantingham and Brantingham , ) all emphasize situational rather than personal causes of crime, and implicitly suggest that they should be measured and analyzed. The Space-Time Budget method is also potentially useful for social disorganization theory (Sampson ), as it allows researchers to replace proxy measures of exposure (e.g., neighborhood of residence) with detailed actual measures of exposure to various environments, including neighborhoods. 2b1af7f3a8